Shim-pack GISS Series - Características
HPLC Column Solutions
Column Chemistries
| Shim-pack GISS C18 | |
| Bonded Phase | Octadecyl Groups |
| Analysis Mode | Reversed Phase |
| Features | High-speed analysis with ultra-high inertness and high durability |
| Particle Size (μm) | 1.9, 3, 5 |
| Pore Size (nm) | 20 |
|
Surface Area (m2/g)
|
200 |
| Carbon Loading (%) | 9 |
| End Cap | ○ |
| pH Range | 1-10 |
| USP Code | L1 |
A highly inert packing material reduces adsorption of basic compounds such as hypertensive drugs and provides good peak shape.
The pore size of 200Å enables good separation of complex and large molecular weight samples such as digested peptides.
High-speed analysis with ultra-high inertness and high durability
Analysis Examples
β-blocker
■Peaks (100 μg/L each)
| Q1 > Q3 | |
| 1. Acebutolol | 337.10 > 116.05 (+) |
| 2. Atenolol | 267.25 > 145.00 (+) |
| 3. Labetalol | 329.00 > 161.95 (+) |
| 4. Nadolol | 310.05 > 254.00 (+) |
| 5. Pindolol | 249.80 > 116.00 (+) |
■Conditions
| Column | Shim-pack GISS C18 (50 mmL x 2.1 mmI.D., 1.9 μm) |
| Eluent | A) 10 mM Ammonium Formate in H2O B) 10 mM Ammonium Formate in CH3OH A/B = 70/30 ‒ 0.3min - 40/60 ‒ 0.5min - 0/100 ‒ 0.1min ‒ 0/100 ‒ 0.01min -70/30 ‒ 0.5min - 70/30, v/v |
| Flow Rate | 0.6 mL/min |
| Col. Temp. | 40 °C |
| Detection | LC/MS/MS（ESI, Positive, Negative MRM） |
| metal-free | Available |