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    Shim-pack GIS Series - Características

    HPLC Column Solutions

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    Column Chemistries

      Shim-pack
    GIS C18    		 Shim-pack
    GIS C18-P    		 Shim-pack
    GIS RP-Shield    		 Shim-pack
    GIS CN    		 Shim-pack
    GIS SIL    		 Shim-pack
    GIS HILIC
    Bonded Phase Octadecyl Groups Octadecyl Groups Octadecyl Groups Cyanopropryl Group - Diol Group
    Analysis Mode Reversed Phase Reversed Phase Reversed Phase Reversed Phase, Normal Phase Reversed Phase, Normal Phase​ HILIC
    Features High retentivity, lower columnback pressure, high inertness High Steric Selectivity Embedded with a Polar Functional Group Suitable in either reversedphase or normal phase mode Suitable in either reversed phase or normal phase mode​ Ideal for the Separation of Highly Polar Basic Compounds
    Particle Size (μm) 2, 3, 4, 5, 10 3, 5 5 3, 5 3, 5 3, 5
    Pore Size (nm) 10 10 10 10 10 10
    Surface Area (m2/g)
    		 450 450 450 450 450 450
    Carbon Loading (%) 15 29 9 14 - 20
    End Cap ○ - - - - -
    pH Range 2-7.5 2-7.5 2-7.5 2-7.5 2-7.5 2-7.5
    USP Code L1 L1 L1 L10 L3 L20

    High retentivity, lower column back pressure, high inertness

    Analysis Examples

    Food Dyes

    ■Peaks

     1. Tartrazine  7.6 mg/L
     2. Amaranth  3.8 mg/L
     3. Ingigocarmine  7.6 mg/L
     4. New coccine  3.8 mg/L
     5. Sunset Yellow FCF  5.3 mg/L
     6. Naphthol Yellow S  7.6 mg/L
     7. Uranine  3.8 mg/L
     8. Allura Red AC  5.3 mg/L
     9. Ponceau R  7.6 mg/L
     10. Ponceau SX  5.3 mg/L
     11. Orange I  5.3 mg/L
     12. Fast green FCF  3.0 mg/L
     13. Brilliant blue FCF  3.0 mg/L
     14. Ponceau 3R  7.6 mg/L
     15. Erythrosine  5.3 mg/L
     16. Azure Blue VX  3.0 mg/L
     17. Orange II  7.6 mg/L
     18. Acid red  3.0 mg/L

     

    ■Conditions

     Column  Shim-pack GIS C18 (150 mmL. x 4.6 mmI.D., 4 μm)
     Eluent  A) 10mM Na2HPO4 (pH6.9 ; 10mM NaH2PO4)
     B) CH3CN
     A/B = 90/10 - 50 min - 65/35
     Flow Rate  1.0 mL/min
     Detection  UV 270 nm
     Col. Temp.  40 °C
     Injection Vol.  10 μL

    Free Amino acids and Organic acids

    ■Conditions

     Column  Shim-pack GIS CN
     (250 mmL. x 4.6 mmI.D., 5 μm)
     Eluent  20 mM KH2PO4 (pH 4.0; H3PO4)
     Flow Rate  1.0 mL/min
     Col. Temp.  40 °C
     Detection  UV 210 nm
     Injection Vol.  5 μL