Analysis of Purified Glucose and Glucose Hydrate in Accordance with the Japanese Pharmacopoeia

Glucose is known as the most abundant sugar in the natural world, and is also used as a pharmaceutical product to take energy parenterally.
Test methods for purified glucose and glucose hydrates (hereinafter referred to glucose) were added to Supplement I to the Japanese Pharmacopoeia, 17th
Edition, which was published in December 2017 (1). In this test method, HPLC method using a refractive index detector is adopted for the purity test and the assay. This article introduces an example of analysis of glucose based on the Japanese Pharmacopoeia.

Chromatograms of Four Sugar Components
Top: System Suitability Solution, Middle: Standard Solution (2), Bottom: Standard Solution